The number of Th17 cells in the spleen and cardiac tissue is decreased by Ad-IL-17AR:Fc.
(A) Representative flow cytometry images of Th17 (CD4+ IL-17+) cells from each group gated on CD4+ T cells. Numbers in the upper right quadrants and lower right quadrants indicate the separate percentages of Th17 cells and CD4+ T cells, respectively. PE = phycoerythrin; FITC = fluorescein isothiocyanate. (B) The percentage of Th17 (CD4+ IL-17+) cells in each group was analyzed using CellQuest software. (C) The levels of IL-6 and TNF-α in the supernatants of heart homogenates detected using ELISA. The data represent the mean ± SEM for 6 mice per group. **P<0.01 vs control group, *p<0.05 vs control group.