The intracellular calcium chelator BAPTA decreased cell retention of I-HIV at 20 uM (panel A) and increased transport rate of I-HIV at 10 uM (panel B).

This is consistent with an increased rate of transport across the BMEC. The calcium channel blocker verapamil had no affect on transport. The calcium ionophore A23187 increased I-HIV permeability (panel C) and decreased TEER (panel D) in BMEC.