The gelatinase activity alterations of MMP-2/9 in both cells were assessed by a gelatin zymography assay.

Gelatin zymography of both CFPAC-1 and MIA PaCa-2 cells was carried out by subjecting conditioned media samples to 10% SDS-PAGE containing 1 mg/mL of gelatin and then stained with 0.25% Coomassie brilliant blue R-250.