The endogenously-generated Th17 response in NO2-promoted allergic airway disease is qualitatively different from the Th17 response generated following Th17 adoptive transfer.
Mice were treated as in Figure 4. Lung single-cell suspensions were restimulated with OVA antigen in the presence or absence of anakinra (Ana; 0.2 μg/mL), Dex (10-8 M), or anakinra and Dex in combination for 96 hours. Cell supernatants of antigen-restimulated and treated lung cells from mice subjected to NO2-promoted allergic sensitization and OVA challenge (A-D) or Th17 adoptive transfer (E-H) were analyzed for the production of cytokines by Milliplex. Statistics were performed by 1-way ANOVA and Bonferroni post hoc analysis. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05 compared to controls (None) unless otherwise indicated by brackets. ND, not significantly different compared to controls. For NO2/OVA sensitized and challenged mice, n = 6. For Th17 and Th2 adoptively transferred mice, samples from n = 3 mice were pooled prior to in vitro culturing, which was performed in triplicate.