The effects of MEHP exposure on interaction of RelB/p52 with the CRH/COX-2 gene promoters.

Term cytotrophoblast were exposed to MEHP for 24 hrs at concentrations as indicated. ChIP assays were performed to determine occupancy of RelB/p52 at CRH (A) and COX-2 (B), and also occupancy of RelA at the CRH (C) and COX-2 (D) gene promoter. Fold enrichment was derived with normalization to a human DNA a satellite, a non-coding DNA sequence to which no transcriptional factors should bind. Rabbit IgG was used as a non-specific control. The bars indicate the average of fold enrichment with error bars representing the standard deviation from three independent experiments. * p < 0.01.