The effect of sulodexide on fibronectin gene and protein expression in renal tissue in control and DN C57BL/6 mice.

(A) Gene expression of fibronectin (FN) in control and DN mice treated with saline or sulodexide as determined by real-time PCR. (B) Representative images of fibronectin expression in control and DN mice at baseline and after 12 weeks treatment with saline or sulodexide are shown. Original magnification x1000. Boxed areas are enlarged to compare glomerular expression of fibronectin (depicted by arrows). Image-based computer assisted analysis was performed to semi-quantify the amount of fibronectin in the (C) glomeruli and (D) tubulo-interstitium of control and DN mice. Results are expressed as mean+SD of data obtained from 6 mice per group. ^§P<0.05, ^§§P<0.01, ^§§§P<0.001 compared to baseline for the same group, ^###P<0.001, DN baseline vs non-diabetic baseline, **P<0.01, ***P<0.001, DN mice vs non-diabetic mice for the same treatment, ^‡P<0.05, ^‡‡P<0.01, ^‡‡‡P<0.001, saline vs sulodexide treatment for the same time-point in DN mice.