The effect of exogenous expression of XHSBP1 on the HSR.

(A) Fluorescence microscope analysis of reporter gene expression from bicistronic plasmids. A6 cells were transfected with the indicated dual reporter gene plasmids based on viral 2A peptides. At 24 h after transfection, GFP and DsRed expression was determined by fluorescence microscope analysis. (B) Western blot analysis of gene expression from bicistronic plasmids. A6 cells were transfected with the indicated 2A peptide-based plasmids. At 24 h after transfection, cell lysates were made and subjected to SDS-PAGE and western blot analysis using anti-GFP antibodies. (C) Western blot analysis of gene expression from bicistronic plasmids. A6 cells were transfected with the indicated 2A peptide-based plasmids. At 24 h after transfection, cell lysates were made and subjected to SDS-PAGE and western blot analysis using anti-GFP and anti-myc tag antibodies. (D) Reporter gene analysis of the effect of native XHSBP1 on the HSR. A6 cells were transfected with mixtures of an Hsp70-luciferase reporter, a CMV-β-galactosidase reporter and the indicated plasmids. Relative luciferase activities and -fold induction were determined as described in the legend to fig. 2C. The results are the average of three independent transfections (standard deviations are indicated by error bars).