The effect of clozapine treatment on mitochondria.

A) membrane potential, B) morphology, and C) volume in SKNSH neuroblastoma cells. SKNSH cells were treated with 0, 10, 20 or 50 µM clozapine for 24 hours and then incubated with TMRM for 20 minutes at 37°C with 5% CO2. Confocal micrographs analyzed for mitochondrial membrane potential and volume using the Nernst Potential MulPro2D plug-in for Image J software.