The effect of TEL, LOS, and ROSI on osteoblastic phenotype of U-33/γ2 cells.

Cells were treated for 3 days with either vehicle (DMSO) (V), or ROSI (R), or TEL (T), or LOS (L), or in combination (RT or RL). A. Enzymatic activity of alkaline phosphatase (ALP) after treatment with different doses of TEL or 1 µM ROSI. B. Relative expression of osteoblast-specific transcription factors, Runx2 and Osterix, in cells treated with either 1 µM ROSI, or 50 µM TEL, or in combination. C. ALP activity in cells treated with either 1 µM ROSI, or 50 µM TEL, or in combination. D. ALP activity in cells treated with either 1 µM ROSI, or 50 µM LOS, or combination. ALP activity was normalized to the number of cells assessed in MTT proliferation assay (panels A, C, and D). * p<0.05 vs. vehicle.