The deletion of BART miRNAs enhances virus production in infected B cells.
(A) This figure shows a western blot analysis performed a different time points on B cells from the same donor transformed with M81, M81/ΔAll or ΔZR with antibodies specific for gp350 and actin. The upper picture shows expression of gp350 and of its alternative spliced form gp220 in these LCLs. The relative intensity of the signals was quantified using the ImageJ software and is depicted in a graph of bars. (B) We generated LCLs by exposing B cells from 6 different donors to M81 or M81/ΔAll. These cells were immunostained with antibodies specific for gp350 as exemplified in the top pictures. The adjacent scatter plot shows the percentage of gp350-positive cells, including cells producing gp350 and B cells covered by virions, in these LCLs at different days post infection. The figure also shows the p values obtained from paired t tests performed with the two types of LCLs. (C) We quantified the EBV DNA load in supernatants from three couple of LCLs obtained by infection with M81 or M81/ΔAll by qPCR and show the results in this scatterplot. The p values of paired t tests performed with the different types of supernatants are indicated. (D) This graph gives the result of B-cell transformation assays that were performed by exposing primary B cells to supernatants from three different LCLs obtained with M81 or M81/ΔAll virions.