The TUNEL staining of the pancreatic islets and the exocrine tissues after cold storage.
Representative of TUNEL positive islets (A), with insulin staining (B), and representative of TUNEL negative islets (C), with insulin staining (D) are shown. (E) The TUNEL positive cells in the exocrine tissues are shown (arrow). (F) The percentage of TUNEL positive islets (the number of TUNEL positive islets/the total number of islets *100) was calculated and evaluated. No significant differences were detected between groups. (G) The density of TUNEL positive cells in the exocrine tissues (the number of TUNEL positive cells/the total area of exocrine tissues in the sections) was calculated. The density of TUNEL positive cells in the ductal injection and combination groups was significantly lower than that of the control group. Magnification ×200. The data are shown as the means ± SE. *P<0.05.