The Expression of Eye Field Transcription Factors subdivides the Optic Vesicle.

A–H: Coronal sections through HH10 optic vesicles immuno-labeled as indicated on panels. A: DAPI stained coronal section, to highlight the relevant tissues. Asterisk indicates the paraxial mesenchyme in contact with the posterior optic vesicle. Green arrow indicates the targeted site of injection for labeling the central neural retina in previous figures. B: Graphic representation of sections with axes labeled for orientation. C–F: Single immunostaining as indicated. G, H: Double immunostaining as indicated. Scale Bar in A applies to panels C–H. Scale bars = 100 µm.