The COX-2/PGE2 pathway is upregulated in BT20 and MCF7 spheroids.

(A) qPCR data showing fold change in COX-2 gene expression in BT20 and MCF7 spheroids in comparison to monolayer cells (n = 3) (B) Western blot data indicating the expression of COX-2 protein in whole cell lysates from BT20 and MCF7 cells cultured as spheroids and monolayer (C) ELISA results for PGE2 level in media conditioned by BT20 and MCF7 cells cultured as spheroids and monolayer (n = 3).