The COX-2/PGE₂ pathway is upregulated in BT20 and MCF7 spheroids.

(A) qPCR data showing fold change in COX-2 gene expression in BT20 and MCF7 spheroids in comparison to monolayer cells (n = 3) (B) Western blot data indicating the expression of COX-2 protein in whole cell lysates from BT20 and MCF7 cells cultured as spheroids and monolayer (C) ELISA results for PGE₂ level in media conditioned by BT20 and MCF7 cells cultured as spheroids and monolayer (n = 3).