Tcf-4 knockdown is more effective at inhibiting cell proliferation and inducing apoptosis than β-catenin knockdown in colon cancer cells.
Cells were treated with adenoviruses (50 MOI) carrying shRNA for 24 h. a, Cell proliferation was determined using a colony formation assay. b, Cell apoptosis was determined using Annexin-V-FITC staining. c, The expression of proapoptotic enzyme caspase-3 was determined by western blot. Each data point represents the mean ± s.d. of three or more independent determinations. *P<0.01 versus control shRNA; #P<0.05 versus β-catenin shRNA; ##P<0.01 versus β-catenin shRNA.