TLR agonist treatment induced IL-10 production in microglia.
The microglial cells were treated with 50 ng/ml of Pam2CSK4 or Pam3CSK4 for 16 hrs. The medium was replaced with fresh growth medium with or without NS3 for another 6 hours. ELISA was performed to detect IL-10 secretion. CHME3 exposed to 20 ng/ml of NS3 for 6 hrs were used as positive control. (A) Microglial cells exposed to Pam2CSK4 or Pam3CSK4 for 16 hrs secreted IL-10 at a significant level (* p < 0.05). (B) IL-10 production was significantly up regulated in Pam2CSK+NS3 (** p < 0.01) and Pam3CSK4+NS3 (## p < 0.01) treated cells compared to NS3 alone treated cells, also IL-10 significantly up regulated in Pam3CSK4+NS3 exposed cells compared to Pam3CSK4 exposed cells (^^ p < 0.01). The data is expressed as mean (n = 3) ± SE.