TLR4 deficiency suppressed inflammatory and fibrotic responses in diabetic kidney.

The expression of endogenous TLR ligands was upregulated in WT-DN kidneys and TLR4−/−diabetic kidneys at week 6 compared with WT non-diabetic controls. (A). Gene expression of IL-6 (A), TNF-α (B), CCL2 (C), CXCL10 (D), TGF-β (E) and fibronectin (F) were upregulated in WT mice with diabetes, but suppressed in TLR4−/− mice with diabetes, as measured by RT-PCR. Data are presented as means ± SD; ** p<0.01; *** p<0.001. The number of animals per group was described in Figure 1.