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Survival of Fasciola hepatica newly excysted juveniles (NEJs) in Fasciola saline (FS) vs RPMI 1640.
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posted on 2014-09-25, 03:33 authored by Paul McVeigh, Erin M. McCammick, Paul McCusker, Russell M. Morphew, Angela Mousley, Abbas Abidi, Khalid M. Saifullah, Raman Muthusamy, Ravikumar Gopalakrishnan, Terry W. Spithill, John P. Dalton, Peter M. Brophy, Nikki J. Marks, Aaron G. MauleNEJ viability in FS is improved both by regular media changes (−change = without media changes; +change = with media changes), or by maintenance in larger volumes (50 µl vs 1000 µl). Experiments were replicated 3 times, employing 15 worms per treatment. Symbols represent mean±SEM.
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RNA interferencevirulence genescontrol target validation27 ntRNAi penetrancedsRNA exposureRNAi persistencetranscriptional suppressionFhe σGSTanthelmintic resistanceJuvenile Fasciola sppprotein knockdown dynamicsCysteine proteases4 h exposureprobe RNAi dynamicsGene functionVitro BackgroundFasciola sppcathepsin LFasciola gigantica juvenilesliver fluke NEJsFhe σGST FindingsRobust transcriptionalVivo Studiesknockdown dynamicssiRNA triggersprotein suppressionliver flukeGene silencingRNAi Dynamicsstandardised RNAi protocolsFasciola hepaticaFheCatL protein suppressionLiver Flukes21 daysliver fluke cause
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