Suppression of cell lysis and 5-FU sensitivity in Δpub1 cells.

(A) L972, UMP31 (Δura4), and KNP32 (Δura4 Δpub1) were grown for 12 h in YES liquid medium. These cells were spotted on YPD or YPD+Ade+Leu medium and incubated for 3 days. For the alkaline phosphatase assay, BCIP was used as described in Fig 1. (B and C) L972 (ura4⁺), KNP16 (Δfur4), KNP25 (Δpub1), KNP87 (Δpub1Δfur4), UMP31 (Δura4), KNP32 (Δura4 Δpub1), and KNP38 (Δura4 Δpub1 Δfur4) cells were grown in YES liquid medium for 12 h. The indicated cells were spotted on EMM (B) or YPD (C) in the presence or absence of 5-FU (1, 5, 50, 100, or 200 μM) and then incubated at 30°C for 3 days. (D) L972 (ura4⁺), UMP31 (Δura4), KNP32 (Δura4 Δpub1), and KNP38 (Δura4 Δpub1 Δfur4) cells were grown in YES liquid medium for 12 h. These cells were spotted on YES, YE, and YPD plates and incubated at 30°C for 3 days. For the alkaline phosphatase assay, BCIP was used as described in Fig 1.