Suppression of Ts-pmy mRNA expression by siRNA1743 caused defects in larval molting.
A. The larvae were electroporated with 8 µM siRNA1743 (a) or control siRNA (b) and incubated for 18 hours. The untreated larvae (c) were used as a negative control. The larvae with cuticle sheath at the end(s) were counted as molting ones. B. The molting percentage for each treatment was calculated. All of the assays were performed in triplicate. *p<0.05 compared with the control siRNA-treated group.