Stopped-flow analysis of SR-1 RW and GroEL R231W: dependence on prebound MDH and GroES.

<p>A. (<i>upper panels</i>) Analysis of SR-1-RW initiated by addition of ATP only (<i>left</i>, “-GroES”) or ATP and equimolar GroES (<i>right</i>, “+GroES”). <i>Black traces</i> indicate GroEL SR-1 RW only, <i>gray traces</i> indicate SR-1 RW with prebound MDH. B. (<i>lower panels</i>) As in A., but performed using GroEL R231W instead of SR-1 RW in each experiment.</p>