Stopped-flow analysis of SR-1 RW and GroEL R231W: dependence on prebound MDH and GroES.
A. (upper panels) Analysis of SR-1-RW initiated by addition of ATP only (left, “-GroES”) or ATP and equimolar GroES (right, “+GroES”). Black traces indicate GroEL SR-1 RW only, gray traces indicate SR-1 RW with prebound MDH. B. (lower panels) As in A., but performed using GroEL R231W instead of SR-1 RW in each experiment.