Fig_6.tif (1.8 MB)
Stimulation of all three PPARs increased the peroxisome number and metabolic function in calvarial osteoblasts.
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posted on 2015-12-02, 04:10 authored by Guofeng Qian, Wei Fan, Barbara Ahlemeyer, Srikanth Karnati, Eveline Baumgart-VogtOsteoblasts were exposed to agonists and antagonists of PPARɑ (Cip, GW6471), of PPARß (GW0742, GSK0660), and PPARɣ (Tro, GW9662). Cell homogenates were analyzed for the protein level of catalase, PEX14, and PEX13 using ɑ-tubulin as housekeeping protein to ensure equal protein loading on the gel. Semiquantitative analysis of the integrated optical signal intensities of the proteins related to ɑ-tubulin with controls set to 1 are shown in numbers directly below the bands.
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PPAR ßPPAR ɣ. Treatmentknockout mouse modelsperoxisomal genesM 3CT cellsPPAR ß antagonist GSK 0660gene expressionMC 3T cellsosteoblast differentiationperoxisome numbercalvarial osteoblastscell typesPPAR family membersperoxisomal compartmentPex 11β gene expressiongene expression patternspprePPAR ß agonist GW 0742
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