Statin treatment and <i>P. aeruginosa</i> infection modulate the host immune response.

<p>A549 cells were treated with 10 µM simvastatin or an equivalent amount of DMSO (VEHICLE) for 24 hours. Untreated and statin-treated cells were subsequently infected with <i>P. aeruginosa</i> PAO1 for 3 hours at MOI 50∶1. The expression of (<b>A</b>) KLF2 (<b>B</b>) KLF6 (<b>C</b>) IL-8 (<b>D</b>) CCL20 and (<b>E</b>) TLR5 was analysed in uninfected cells, and in the presence of vehicle, simvastatin (SIM), <i>P. aeruginosa</i> (PAO1) and simvastatin and PAO1 combined (SIM+PAO1). *<i>P</i>, ≤0.05, **<i>P</i>, ≤0.01, ***<i>P</i>, ≤0.001.</p>