Statin treatment and P. aeruginosa infection modulate the host immune response.
A549 cells were treated with 10 µM simvastatin or an equivalent amount of DMSO (VEHICLE) for 24 hours. Untreated and statin-treated cells were subsequently infected with P. aeruginosa PAO1 for 3 hours at MOI 50∶1. The expression of (A) KLF2 (B) KLF6 (C) IL-8 (D) CCL20 and (E) TLR5 was analysed in uninfected cells, and in the presence of vehicle, simvastatin (SIM), P. aeruginosa (PAO1) and simvastatin and PAO1 combined (SIM+PAO1). *P, ≤0.05, **P, ≤0.01, ***P, ≤0.001.