Stability of SULT4A1 protein in IMR-32 cells.
Cells were transfected with plasmids for FLAG-tagged wild-type or variant SULT4A1 and then treated with 10 µg/ml cycloheximide for the indicated times. Proteins were then Western blotted using anti-FLAG antibody and anti-tubulin antibody as a loading control. Data are normalized to protein levels at time 0. All results are mean ± s.e.m, n = 4.