Spread colony phenotype of 3610 spontaneous mutants.

A. Differences in colony morphology between 3610 (left) and SPR, a spontaneous spread mutant (GP305, right) growing on LB. The picture in the middle corresponds to a 3610 colony with two branches of spread mutants (arrow). Bar represents 1 cm. B. eDNA production of wild type (3610) and several isogenic spread mutants: SPR-1 (GP305), SPR-2 (GP306), SPR-3 (GP307) and SPR-4 (GP319). Strains were grown in MSgg at 37°C with aeration (growth was similar for all the strains). eDNA refers to the eDNA concentration in the culture supernatant. Data presented are representative of results obtained in two independent experiments.