Spectral density, AABUF and normalized cross-peak intensity analysis.
(A) Plots of J(0) values (vertical bars with error) and AABUF (Average Area Buried upon Folding) (filled black circles). Horizontal bars on top of the plot indicate the regions with AABUF value more than the average. (B) Normalized cross-peak intensities derived from 2D [15N-1H]-HSQC of M-crystallin at different denaturant concentrations. The folded secondary structural elements (0 M GdmCl) are shown on the top of panel.