Specific vs. non-specific binding to epitope peptides of cj0669.
The mean relative fluorescence intensities of peptides 44, 45, Rabbit IgG and MBP are compared after incubation with three different specific anti-Campylobacter jejuni antibodies to anti-E.coli antibodies. Error bars were calculated by propagation of uncertainty by Gauss. Peptides 44 and 45 of cj0669 show mean rfi values of approximately eight and six thousand A.U. respectively after incubation with polyclonal anti-Campylobacter antibodies. However, using E.coli antibodies the signals are almost gone amounting to less than 500 A.U. in rfi. This indicates a predominantly specific interaction of anti-Campylobacter antibody and peptides tested.