Figure_3.tif (1.59 MB)
Specific localization of FABP4 in neovascular endothelial cells.
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posted on 2014-05-06, 02:57 authored by Magali Saint-Geniez, Elisa Ghelfi, Xiaoliang Liang, Chenwei Yu, Carrie Spencer, Stephanie Abend, Gokhan Hotamisligil, Sule Cataltepe(A) Confocal imaging of neovascular tufts in P17 WT OIR retina flat-mount following immunostaining for FABP4 (red), EC-specific marker EMCN (green), activated microglia-specific marker Iba1 (white) and DAPI (blue). FABP4 is strongly associated with the neovascular tufts and absent from the normal vasculature. FABP4 was not detected in the Iba1- positive cells but co-localized with EMCN-positive cells. (B) Immunostaining of FABP4 (red) EMCN (green) and DAPI (blue) in a large neovascular structure followed by confocal sectioning confirmed FABP4 localization to ECs of pathological neovessels. Scale bar is 50 µm.
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anatomybiological tissueepitheliumEpithelial cellsEndothelial cellsCardiovascular anatomyBiochemistrylipidscell biologyCellular typesMolecular cell biologydevelopmental biologyOrganism developmentgeneticsgene expressioncardiologyophthalmologyRetinal disorderspediatricsChild developmentlocalizationfabp4neovascularendothelial
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