Single feeding delays phase of liver, but not lung clock.

After a night-time restricted feeding schedule (ZT12-24) for one week, mice were divided into 4 groups and then delayed feeding was applied as follows. Mice in the 4, 8 and 13 h delayed groups were fed from ZT16, 20, and the next ZT1, respectively. Control mice were fed from ZT12 to ZT24. White and black boxes indicate day and night respectively; gray lines show feeding times (A). Representative circadian profiles of reporter luminescence in explants of livers (B) and lungs (C) isolated from mPer2::Luc knockin mice. Peak times of circadian oscillation between 12 and 36 h in culture are shown for liver (D) and lung (E). Values are shown as means ± SEM (n = 7 for ZT12 and ZT16, and n = 5 for ZT20 and ZT1; *p<0.05, ***p<0.001; vs. control; Dunnett's test).