Simultaneous triple labeling with different combinations of three mAbs in rat and mouse brain.

Sections from rat (A–D) and mouse (E–H) were simultaneously labeled with three different mAbs, and SCS 2°Abs. (A) Ankyrin-G (green), Caspr/Paranodin (red), and Kv1.2 (blue), in a region of rat hindbrain white matter with myelinated axons containing nodes of Ranvier. (B) BK channel (green), GFAP (red), and Kv1.2 (blue), in a region of rat cerebellar cortex. Labels mark the molecular layer (ML), Purkinje cell layer (PCL), and granule cell layer (GCL). (C) Ankyrin-G (green), Kv2.1 (red), and Kv1.2 (blue), in rat hippocampal dentate gyrus. Labels mark the middle molecular layer (MML), inner molecular layer (IML), granule cell layer (GCL), and hilus (HI). (D) Caspr/Paranodin (green), Ankyrin-G (red), and Kv1.2 (blue), in rat hippocampal dentate gyrus, labels are as in Panel C. (E) mAbs and 2°Abs as in A, but in mouse hindbrain white matter with myelinated axons containing nodes of Ranvier. (F) Kv1.2 (green), Ankyrin-G (red), and GFAP (blue), in a region of rat cerebellar cortex. Labels as in B. (G) Ankyrin-G (green), KChIP1 (red), and Kv1.2 (blue), in mouse cerebral cortex. (H) Kv2.1 (green), Kv1.2 (red), and PSD-95 (blue), in mouse hippocampal dentate gyrus. Labels as in D. Scale bars: A, E, F = 10 µm; B = 20 µm; C, D, G, H = 50 µm.