Silencing of endogenous c-Myc reduces MYCT1-TV promoter activity.

A. Detecting of c-Myc expression level in cells by qRT-PCR. The expression of GAPDH was used as an internal control. Hundred nanogram of no siRNA (Mock), nonspecific control siRNA (NC) or c-Myc specific siRNA (c-Myc) were transfected into Hep2 (black bars) or HEK293 (gray bars) cells. B. Luciferase activity of MYCT1-TV promoter after transfected with c-Myc siRNA. Luciferase activity was measured in Hep2 (black bars) or HEK293 (gray bars) extracts 48 h after transfection. pGL3-Basic, cells cotransfected with pGL3-Basic and pRL-TK; P852, cells cotransfected with P852 and pRL-TK; P852+NC, cells cotransfected with P852, NC siRNA and pRL-TK; P852+c-Myc, cells cotransfected with P852, c-Myc siRNA and pRL-TK. Data are indicated as the means ± SEM of three independent experiments.