Serologic and perturbed gene expression in time-shifted data.

(A) Log10 plaque reducing neutralizing titers (Log10(PRNT₈₀)) of test calves prior to and after vaccination with arMP-12 with data synchronized to the time point that each animal reached the minimum threshold (T = 0; 1:80, log10 = 1.903). (B) Schematic illustrating the immunologic status of test animals in time shifted analysis: unvaccinated (gray), vaccinated, below PRNT₈₀ cutoff (light red), vaccinated, first day at or above PRNT₈₀ cutoff (dark green), vaccinated, above PRNT₈₀ (light green). (C) Count of significantly perturbed genes normalized against unvaccinated samples occurring in at least 50% of animals by time pre-serum neutralization, with a |Z-score ≥2.24| and a false discovery rate (FDR) ≤0.05.