Sensitivity of the Total DnaK to the Change in the Transcription Rate Constant for DnaK at Low Temperature

The plot reports the sensitivity computed for the parameters values that satisfy the required yield and efficiency (yield > 0.9 and efficiency > 0.85 at low and high temperature). Yield and efficiency were computed by searching the 2-D parameter space consisting of the transcription rate constant for σ32 and the association constant between σ32 and DnaK as follows:

for the various systems,

(A) DEG-FB/FF double mutant (SEQ-FB system): K[5] = K[6] = 0 M−1, kx[1] = kx[2] = 0 min−1, η = 1,

(B) FF mutant (SEQ-FB + DEG-FB system): K[5] = K[6] = 1 × 108 M−1, kx[1] = kx[2] = 5 min−1, η = 1,

(C) Wild-type (SEQ-FB +DEG-FB + FF system): K[5] = K[6] = 1 × 108 M−1, kx[1] = kx[2] = 5 min−1, η = 1 (low), η = 2 (high).