Secretion and surface exposure of PE-based GFP chimeric proteins in M. smegmatis and M. bovis BCG.

Pellet (P) and culture supernatant (S) analysis was carried out on M. smegmatis (A) or M. bovis BCG (B) strains expressing different fusions of the PE domain of PE_PGRS33 with GFP. Proteins were detected by Western blot using monoclonal antibodies against GFP or GroEL.