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Schematics of the domain structure in three variants of SACTE_2347.

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posted on 2014-04-07, 03:25 authored by Taichi E. Takasuka, Justin F. Acheson, Christopher M. Bianchetti, Ben M. Prom, Lai F. Bergeman, Adam J. Book, Cameron R. Currie, Brian G. Fox

A, SACTE_2347_FL; B, SACTE_2347_42kDa; C, SACTE_2347_34kDa. Residues 1–41 correspond to the annotated twin arginine translocation peptide. Residue 51 corresponds to the experimentally determined N-terminus in the three variants. The GH5 domain spans residues 63–308, and boundaries of the Fn3 and CBM2 domains are as indicated. The location of C-terminal tryptic-digest polypeptides for each variant is shown (GH5, 34kDa-C, Fn3, 42kDa-C, CBM2; also shown in Table 2 and Figure S1).

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