Figure_7.tif (190.04 kB)
Schematic representation of the +1-peuA′-flag (A) and +39-peuA′-flag (B) DNA fragments.
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posted on 2014-08-22, 03:34 authored by Tomotaka Tanabe, Ayaka Kato, Keiichi Shiuchi, Katsushiro Miyamoto, Hiroshi Tsujibo, Jun Maki, Shigeo Yamamoto, Tatsuya FunahashiEach of these DNA fragments includes a nucleotide sequence corresponding to the peuA 5′-UTR from the +1 or +39 sites and the nucleotide sequence for the N-terminal 99 amino acid residues (in gray), in addition to a T7 promoter and a FLAG tag preceding the stop codon (TAA). The secondary structures of the 5′-UTRs of the +1 transcript (A) and the +39 transcript (B) of peuA are shown, both of which were predicted by the CentroidFold software (http://www.ncrna.org/centroidfold/). The RBS and start codon of peuA mRNA are boxed in the secondary structures.
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pH 8.0pH 7.0translation stagePeuAcomplementation experimentsSecondary structure predictionVibrio parahaemolyticus peuA Gene Encodingsilico analysesPrimer extension analysisVibrio parahaemolyticusferric uptake regulatorTonB 2 system genestranslation analysisdeletion mutantspeuRS deletionnovel gene regulation mechanismenterobactin utilizationsystem operonundescribed receptorpeuA expressionsitevpatranscriptTranslation initiationAlternative Ferric Enterobactin Receptorsystem PeuRS
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