Schematic representation of Tetra-Primer Amplification Refractory Mutation System.

T-ARMS-PCR was used for the detection of SNPs of Fas rs1800682 (A), Fas rs2234727 (B), FasL rs763110 (C) and FasL rs5030772 (D). Two forward and two reverse specific primers are used to produce three potential products. Product sizes were 158 bp for A allele, 207 bp for G allele, and 309 bp for two outer primers (control band) for Fas rs1800682 (A). Product sizes were 216 bp for G allele, 340 bp for A allele, and 507 bp for control band for Fas rs2234727 (B). Product sizes were 145 bp for T allele, 192 bp for C allele, and 284 bp for control band for FasL rs763110 (C). Product sizes were 197 bp for G allele, 300 bp for A allele, and 438 bp for control band for FasL rs5030772 (D).