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SYBR green real-time PCR primers for the identification and quantification of the NA and NS genes containing or not containing amino-acid deletions in the viral cDNAs.

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posted on 2014-04-17, 03:07 authored by Yanfang Li, Sujuan Chen, Xiaojian Zhang, Qiang Fu, Zhiye Zhang, Shaohua Shi, Yinbiao Zhu, Min Gu, Daxin Peng, Xiufan Liu

NA-Fa, the primer was targeted to the nucleotide sequence of the 20-amino-acid insertion in the NA stalk.

NS-Fb, the primer was targeted to the nucleotide sequence of the five-amino-acid insertion in the NS1 protein.

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