SDS-PAGE of recombinantly expressed fusion constructs.

The whole lysates labelled by incubation with HaloTag® Alexa 488 ligand were separated by SDS-PAGE. M refers to the BenchMark™Fluorescent Protein Standard (Invitrogen). For almost all proteins, bands were visible with the respective sizes of their molecular weight and the HaloTag™. The HaloTag™ alone at 34 kDa is visible throughout. The proteins were as follows: 1 – cj0476, 2 – cj1723, 3 – cj1208, 4 – cj1619, 5 – cj0920c, 6 – cj1380, 7 – cj1381, 8 – cj0669, 9 – cj1624, 10 – cj1320, 11 – cj1486, 12 – cj0130, 13 – cj1366, 14 – cj0016, 15 – cj1575, 16 – 1576, 17 – cj1729, 18 – cj0571, 19 – cj0926, 20 – cj0927, 21 – cj0623 and 22 – cj1138. The pictures of the two gels as well as the righthand marker were fused by CorelDraw to minimize space. For all proteins except cj0920c (5) and cj1320 (10) bands are visible. In lane 4, cj1619 shows a band at around 65 kDa whereas the fusion protein ought to show a size of approximately 80 kDa. The correct-sized fusion proteins are highlighted in red.