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SDS-PAGE of cells expressing Nef (A) or myristoylated Nef (myr.Nef) resulting from coexpression of Nef and hNMT (B).

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posted on 2013-02-21, 01:50 authored by Julian M. Glück, Silke Hoffmann, Bernd W. Koenig, Dieter Willbold

Aliquots of the expression cultures were taken before as well as 1.5 and 5 hours after IPTG induction. The corresponding lanes are labeled with “B”, “1.5” and “5”, respectively. In addition, protein molecular weight marker (“M”) was applied.

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