SDS-PAGE of cells expressing Nef (A) or myristoylated Nef (myr.Nef) resulting from coexpression of Nef and hNMT (B).
2013-02-21T01:50:59Z (GMT) by
Aliquots of the expression cultures were taken before as well as 1.5 and 5 hours after IPTG induction. The corresponding lanes are labeled with “B”, “1.5” and “5”, respectively. In addition, protein molecular weight marker (“M”) was applied.