RickA expression of rickettsiae grown in different cell lines.

(A) Vero cells (V) or L929 cells (L) infected with R. raoultii (Rra) or R. conorii (Rco) were subjected to SDS-PAGE and then transferred to a nitrocellulose membrane. The membranes were subsequently probed with anti-RickA or anti-Adr1 antibodies. Identical amounts of proteins were loaded in each lane. Bartonella quintana (Bq) whole extracts were used as a negative control. (B) Quantification of RickA expression normalized to Adr1 expression for each sample. Data are mean+/−SEM for three distinct experiments.