Resveratrol activates the Akt/mTOR signaling pathway in BV-2 microglial cells.

Panel A shows that BV-2 cells were treated with resveratrol (50 µM) for the indicated times, Panel B shows that BV-2 cells were pre-treated with resveratrol (50 µM) for 1 h, then exposed to LPS (1 µg/ml) for the indicated times and Panels C and D shows that BV-2 cells were pre-treated with resveratrol (25, 50, and 100 µM) for 1 h, then exposed to LPS (1 µg/ml) for 40 min. Various treated BV-2 cell lysates (50 µg protein) were prepared and subjected to Western blot analysis by using antibodies specific for PTEN and phospho-PTEN, Akt and phospho-Akt, mTOR and phospho-mTOR as described in the methods. The relative protein levels were quantified by scanning densitometry and normalized to total Akt. The values shown are mean ± SEM of data from three independent experiments. ^#Significant compared with control alone, p<0.05. *Significant compared with LPS alone, p<0.05. ^▵Significant compared with resveratrol+LPS, p<0.05.