Representative images of bi-dimensional DIGE of protein extracts from Brucella ovis wild type and ΔabcBA mutant.

(A) Enumerated spots were differently expressed (p<0.05) between ΔabcBA and WT strains and selected for mass spectrometry. Spots with lower expression were excised from B. ovis WT gel (left panel), whereas spots with higher expression were excised from B. ovis ΔabcBA gel (right panel). (B) Topographic 3D images of the signal intensity correspond to a lowly expressed (left) and a highly expressed (right) spot.