Representative confocal fluorescence micrographs of NRVMs grown on cover glasses.
Ad-GFPu infection and BFA (10 nM) treatment are as described in Figure 4. At 24 hr after BFA treatment, the cells were fixed with 4% paraformaldehyde and subject to immunofluorescence staining for p62 (red). GFPu direct fluorescence (green) and p62 indirect immunofluorescence were visualized and captured using a confocal microscope. In the BFA treated cells, both p62 and GFPu are increased and the increased GFPu is co-localized with p62. Scale bar = 50 µm.