Figure_6.tif (98.64 kB)
Relative levels of peuA mRNA, assessed by RT-qPCR.
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posted on 2014-08-22, 03:34 authored by Tomotaka Tanabe, Ayaka Kato, Keiichi Shiuchi, Katsushiro Miyamoto, Hiroshi Tsujibo, Jun Maki, Shigeo Yamamoto, Tatsuya FunahashiTotal RNA samples were prepared from VPD54 (vctA- and irgA-deficient mutant derived from VPD5) and VPD102 (peuRS-deficient mutant derived from VPD54) grown at pH 7.0 and 8.0 in LB-Tris, LB-Tris/+EDDA, and LB-Tris/+EDDA/+Ent media. Data are shown as means ± SD from 3 separate experiments. An asterisk indicates P<0.05 compared to other samples.
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pH 8.0pH 7.0translation stagePeuAcomplementation experimentsSecondary structure predictionVibrio parahaemolyticus peuA Gene Encodingsilico analysesPrimer extension analysisVibrio parahaemolyticusferric uptake regulatorTonB 2 system genestranslation analysisdeletion mutantspeuRS deletionnovel gene regulation mechanismenterobactin utilizationsystem operonundescribed receptorpeuA expressionsitevpatranscriptTranslation initiationAlternative Ferric Enterobactin Receptorsystem PeuRS
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