Reduced Th2 cytokine production after in vitro restimulation of thoracic cavity cells of acute, but not chronically L. sigmodontis infected ST2-ko mice.
Isolated cells from the thoracic cavity of individual L. sigmodontis infected wild type (WT) or ST2-ko mice were cultured in vitro with either L. sigmodontis antigen (LsAg, left panel) or ConA (right panel). IL-4 (A,B), IL-5 (C,D), IL-10 (E,F), IFNγ (G,H), IL-33 (I,J) and IL-25 (K,L) within the culture supernatants were measured on days 35, 60 or 100 post infection. Data is representative for two independent experiments with at least 5 mice per group. Differences were tested for statistical significance by Mann-Whitney-U-test, *p<0.05, **p<0.01.