Recruitment profiles under derepressed conditions.
2013-02-21T11:17:39Z (GMT) by
<p>A–G: ChIP analysis for coactivators/general transcription machinery at the indicated promoter regions in a wildtype strain (black), <i>adr1Δ</i> (pink), <i>cat8Δ</i> (blue) and <i>adr1Δcat8Δ</i> (green). Binding is expressed as the percent of the wildtype derepressed value (set to 100%) after normalization to the <i>TEL</i> negative control locus. Error bars represent the standard deviation of biological replicates (two or more). Data is shown at 4 hours of derepression. H: ChIP analysis for Taf1 in repressing (grey bars) and derepressing (four hours, black bars) conditions. Error bars represent technical replicates. Inset: ChIP analysis by PCR at for Taf1 at <i>ACT1</i>. The protein(s) assayed for in each case is listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0001436#pone-0001436-t002" target="_blank">Table 2</a>.</p>