Recognition of rTc_11623.20 and rTc_N_10421.310 by the sera from chronic chagasic patients or Leishmania-infected individuals.

Sera from chronic chagasic patients and sera from patients with cutaneous and visceral leishmaniasis were assayed by ELISA using rTc_11623.20 (A, D), rTc_N_10421.310 (B, E) and pooled recombinant proteins (C, F). Tc-non typed, sera from chronic chagasic patients; TcII, sera from patients infected with T. cruzi TcII DTU; TcVI, sera from patients infected with T. cruzi TcVI DTU, Tc-Ind, sera from chagasic patients in the indeterminate form of Chagas disease; Tc-Card, sera from chagasic patients in the cardiac stage of Chagas disease; CL, sera from patients with cutaneous leishmaniasis; VL, sera from patients with visceral leishmaniasis; C-, uninfected individuals. Squares represent sera from chagasic patient in the initial cardiac stage. The dotted line represents the cutoff value that was obtained based on the ROC curve. The solid line corresponds to the mean values.