Re-expression of Pyk2 stimulates migration of miR-23b overexpressing cells.
A. T98G cells transduced with empty vector (ctrl) were left uninfected while T98G cells overexpressing miR-23b were infected with adenoviruses encoding ß-galactosidase, or FLAG epitope-tagged wild-type Pyk2 (Pyk WT) or Pyk2 lacking the 3' UTR (Pyk 3Kb) at MOI = 5. Sixteen hours after infection, cells were plated onto laminin coated slides and the migration rate determined after 24 hours. Values are mean ± SD of 10 replicate samples (*: p<0.05). B. Whole cell lysates were immunoblotted with the indicated antibodies.