RT-qPCR for CBR.1. CBR.2, CYB5 and crtR transcript levels in the wild-type and mutant strains.

(A) CBR.1 (B) CBR.2, (C) CYB5 and (D) crtR genes. The transcript level of each gene was normalized to actin mRNA. Black and grey bars represent the results using RNA extracted after 36 and 72 h of culture, respectively. Values are the mean ± standard deviation of two technical replicates from three independent cultures (Student’s t-test was performed to compare each mutant to the parental strain. * P <0.05 and ** P <0.01).